(2014). The synthesis of plastocyanin (Cu) and Cyt c6 (Fe) was found to be reciprocally dependent on Cu nutrition in both cyanobacteria (Prochlorothrix hollandica, Anabaena, and Synechocystis spp.) A recent transcriptome study identified an additional example of Cu sparing in C. reinhardtii (Castruita et al., 2011). three-dimensional structure of oxidized poplar ( Populus nigra) plastocyanin has been determined by X-ray crystallography at 2.7 A resolution [1] and refined to 1.33 A [2]. One thought is that commitment to plastocyanin, as noted in a diatom, is an adaptation to economize on Fe (Peers and Price, 2006). The evolution of plastocyanin therefore facilitated photosynthesis but increased the dependence on Cu. Iron is transported by transferrin whose binding site consists of two tyrosines, one aspartic acid and one histidine. Obviously, there exist a number of internal discrepancies within the studies on the Chlamydomonas mutants which must be settled before these mutant forms can be so indiscriminately and authoritatively employed to localize the site of action of plastocyanin. (2018b) measured spectral dynamics of PC rods (also without any core) isolated from the ΔAB mutant of Synechocystis; this mutant has PBS with rods, but without core (Ajlani et al., 1995). Like plastocyanin from other species, it is an antiparallel β-barrel possessing a single copper atom, which is coordinated by two imidazole nitrogens (His37 and His87), a thioether … (2000) The Protein Data Bank. Reviewed-Annotation score: -Experimental evidence at protein level i. Plastocyanin, ein Kupferprotein, das bei den Lichtreaktionen der ⇒ Photosynthese als Redoxsystem in der Elektronentransportkette dem Photosystem I (P700) Elektronen vom Cytochrom bf Komplex zuleitet c 6 oxidation contributes to the NIR absorption changes. Retrouvez la définition du mot plastocyanine dans notre dictionnaire en ligne par la-conjugaion.fr. (2018b) have suggested that the red-shifted chromophores (i.e., those with maxima at 672 nm) may be involved in direct EET to PSI in the PBS-PSI supercomplexes, as reported by Kondo et al. An electron-carrying protein present in chloroplasts, forming part of the electron transport chain. It was surprising to find these organisms containing the protein plastocyanin because the concentration of copper dissolved in the ocean is usually low … PSI complexes in cyanobacteria form trimers that are in equilibrium with PSI monomers, which vary as a function of concentration of cations (Kruip et al., 1994) or protons (Schwabe et al., 2001); this aspect of cyanobacteria is quite different from that in plants and green algae, where PSIs exist only as monomers. The presence of plastocyanin in plants is correlated with green, chloroplast-containing cells as is to be expected from its subcellular location in the chloroplast thylakoid lumen. Use of antibodies to block photosynthetic reactions generally dependent on plastocyanin; b. epr spectral assessment; c. treatment of chloroplasts with KCN and amphotericin B ; d. light-induced changes at 553 nm (= cytochrome f photooxidation). Plastoquinone and plastocyanin are mobile electron carriers in the chloroplast electron transport chain. 1, is formulated principally upon the evidence offered from studies on a plastocyanin-deficient mutant of Chlamydomonas (strain ac-208) and on PS I particles isolated from chloroplast by detergent treatment (Trebst and Elstner, 1965; Wessels, 1968; Hind, 1968). plastocyanin. plastocyanin noun Etymology: Greek plasto s + English cyan-+ 1-in Date: 1961 a copper-containing protein that acts as an intermediary in photosynthetic electron transport. As in Photosystem II, light is harvested by antenna complexes, and the primary light reaction is a charge separation beginning stabilized by transfer of an electron to a quinone, but in Photosystem I the terminal electron acceptor is an FeS cluster, which permits reduction of ferredoxin. plastocyanin, P700, S. Katoh, A. San Pietro, A. Takamiya, P.M. Wood Abstract A review is presented of the early history of investigationsinto the function of the blue copper-proteinplastocyanin in photosynthesis. Degradation of plastocyanin is important for Cu recycling as evidenced by the growth phenotype of crr1 mutants, which cannot maintain Cyt oxidase because they cannot recycle Cu from plastocyanin (J. Kropat and S. Merchant, unpublished). noun Etymology: Greek plastos + English cyan + 1 in Date: 1961 a copper containing protein that acts as an intermediary in photosynthetic electron transport Both proteins were present in cells grown in 0.3 MM copper. Page and S. S. Merchant, unpublished). Because of the extremely varied opinions as to the precise site of function of the copper-protein in photosynthesis, it would seem that more extensive studies would have been made with ac-208 by Levine or by other investigators of photosynthesis. It should be recalled that in addition to the ac-208 strain of Chlamydomonas, Gorman and Levine reported on a suppressed strain of ac-208, which although lacking plastocyanin, still performed photosynthesis at approximately 50% of the rate of the wild-type strain. Obtain the plastocyanin coordinates (2PLT) and measure the distance of the copper ion to the closest protein surface. We regard the reaction as closely related to a segment of the normal electron transport chain in vivo. The plastocyanin content of etiolated bean leaves (Phaseolus vulgaris L.) was measured, and the development of the protein in response to light was followed. 3Cu(OH)2) and copper hydroxide (Cu(OH)2), which has often led to more than 10-fold increases in soil Cu. can use two different electron donors to PS I: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Plastocyanin, electron transfer in plastid, CoproporphyrinogenIII oxidase, plastid heme synthesis, Coproporphyrinogen III oxidase, plastid heme synthesis, Aerobic oxidative cyclase, tetrapyrrole biosynthetic pathway, Transcription factor, regulation of Cu deficiency response. (1972) demonstrated rather convincingly that plastocyanin is present both in the grana and the stroma membranes of spinach chloroplasts. a The structure and definition of each metal-ligand distance is shown in the figure below. While plastoquinones are lipid-soluble and therefore move within the thylakoid membrane, plastocyanin moves through the thylakoid lumen. No detectable amount of plastocyanin was present in the cytoplasmic or in the membrane fraction. QED. Mots en P à proximité: plaste, plasticité du sol, plasticité phénotypique, plastidome, plastifiant, plastocyanine, plastoglobule, plastoquinone, plastron, plateau continental, plateau de fruits de mer. Northern analysis of total RNA, probed with … were made by quantitative extraction of plastocyanin and a sensitive assay with an 02 electrode. Electrons enter and leave PC via the H87 ligand to the copper, which is located on the surface of the PC molecule (Figures 1 and 2). We use cookies to help provide and enhance our service and tailor content and ads. Figure 1. 2. When soluble photosynthetic electron transfer catalysts were isolated from a cyanobacterial bloom in the Potomac, Cyt c6 was abundant, but when the organisms were cultured in the laboratory (in a medium amended with Cu), plastocyanin could be isolated (D. Krogmann, personal communication). Plastocyanin is a copper-containing redox protein within the photosynthetic electron transport system. Plastocyanin is a soluble copper-containing protein present in the thylakoid lumen, which transfers electrons to photosystem I. Copyright © 2021 Elsevier B.V. or its licensors or contributors. Recent studies by Baszynski et al. In brief, the acetate-requiring phenotype of pcy1-ac208 could be suppressed by growth on a medium lacking Cu, which induced the expression of the heme-containing cytochrome. The regulatory pathways (i.e., Cu sensor, cis-acting sequences and DNA-binding proteins) are not yet elucidated, but the petJ gene (encoding the Cyt) responds transcriptionally to Cu nutrition status while the petE gene (encoding plastocyanin) responds transcriptionally in some cases and posttranscriptionally in others (Briggs et al., 1990; Bovy et al., 1992). PETE. Both proteins were present in cells grown in 0.3 microM copper. In all cases the binding site is generally conserved. la photosystème I (ou plastocyanin-ferrédoxine oxydoréductase, souvent désigné par l'acronyme PSI) Il est la deuxième photosystème impliquée dans les réactions photosynthétique de algue, plantes et quelques-uns bactéries. Select a category... Plastocyanin Cytochromes f Cytochromes c6 Photosystem I Protein Complex Cytochromes Spinacia oleracea Nostoc Prochlorothrix Electron Transport Copper Plants Photosynthetic Reaction Center Complex Proteins Plant Proteins Chlorophyta Cytochrome b6f Complex Azurin Chloroplasts Cyanobacteria Chlorophyll Oxidation … In an Fe-replete situation (when ferroxidase expression is repressed), the Cu quota is determined largely by plastocyanin and Cyt oxidase. Chlamydomonas can use two different electron donors to PS I: plastocyanin or cytochrome (cyt) c6. When discovered, it was noted to be absent from carrot and turnip roots, and the white underground stems of Japanese scallion, but present in leaves where a high The protein is monomeric, with a molecular weight around 10,500 Da, and 99 amino acids in most vascular plants. Structures of the protein from poplar, algae, parsley, spinach, and French bean plants have been characterized crystallographically. Table 5. The Cu atom is coordinated to four amino acid residues (H87, H37, S84, and M92) in a distorted tetrahedral geometry. Moreover, plastoquinone is present in inner thylakoid membrane, while plastocyanin is present in the thylakoid lumen. It can also function in a cyclic electron transport pathway. The primary electron donor, P700, is ultimately reduced by plastocyanin or cytochrome c6. In addition, these rods have been shown to switch between two spectrally different conformations (with maxima at 651 or at 672 nm). It is a member of plastocyanin family of copper binding… Recently, Gwizdala et al. Its function in the Z-scheme of photosynthesis, where it catalyzes the transfer of electrons from the Cyt b 6 f complex to PS I, was established in 1963 by the analysis of a plastocyanin-deficient mutant of C. reinhardtii ( Gorman and Levine, 1965 ). Plastocyanin is a soluble copper-containing protein present in the thylakoid lumen, which transfers elec-trons to photosystem I. 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