2. SMEAR PREPARATION 1. samples shipped to the laboratory or collected after hours, always make 2-3 peripheral blood smears. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, 27607 Introduction Although tremendous advances have been made in the field of point-of-care hematology analyzers, examination of a well prepared, well stained blood smear remains the … Gravity. Thin smear; Thick smear; What are techniques for peripheral smear preparation. h�d����0@en&Ӥ6����(\֋��i�ؑ���-������Uo`a�-���Ī��Z�Ł1H����ޔ֭�s�++_���/�]�R�Ԝ;N�&����l�7�)uq��?����)N��wLm��()gdp�X��K"=ǫ��G���ft����8���pO��1� �Ghp^�C��L�|��&R�w�o ��L endstream endobj 107 0 obj <>stream Peripheral Smear Preparation. Thin blood film 2. A second glass slide is used as a spreader, streaking the blood … Too slow a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end of the slide. 4. Clinical Laboratory Hematology 2014-2015. Blood smear preparation is a technical skill that requires practice. g��z��x������@ +��(�����q�����on�yd��Sٔ��7Ô)�r\��P�;�s��h����a�c�o�(_�y�����a��/����,V�ݿ�1���|�7���m ��������=�կ#]��w��|=d��j�o�q�m�o8�%�r^�ݺ�.P��]���Z ���yȕ`mg��&W6��_:X�n�Y�Y���������Ö:�{0��t^`:�Rq��E����sGN���UYo�]ݑ������>�F�]��/i4u+0l_��-��p�f(��_n Part 1 - Preparation of Peripheral Blood Smear (PBS) - YouTube Hello. Coverslip smears result in more equitable distribution of leukocytes. The wedge smear is a convenient and commonly used technique for making peripheral blood smears. �Ֆ8�7�Xq0����}��R���sv�`"8'Ѳй��_̢������y�Z��/�J�J+TXԜ��W=��2B�dؚ-"�F�t�t= This method produces a gradual decrease in thickness of the blood from thick to thin ends with the smear terminating in a feathered edge approximately 2 mm long. Deep learning emerges as a promising technology for automated peripheral blood smear analysis and hematologic diagnosis. It is good practice to prepare two slides for each sample. Result: Schistocytes were observed in approximately 78% of normal individuals with a mean of 0.15% of all RBCs. Smear Preparation. Thin smears. Step 3. The spreader slide has been brought in contact with the drop of blood … Well-made smears are required for reliable identification and evaluation of peripheral blood cells. x�]ے$�q}�������u�궟vW����NX������Έ���^����������rP�U�hrB!VOH$2O& ������hʝ�����lj�v[ӵպ1�����|��Si�2��O�Q������n��~�7h��m6e��{���]����|q{[�KS��f��s��y}��ҫjO�ٯ��3ҫ������̛�O,}������b�M���k�*R �;~��+�������@�?��d��P�vlv�tc����vF]���5׈ne�j-��v\��\�[Hߴ�Z�LY��m� �@�F#K�c�)���c�"#g�ݖ�mف�k�*�h6��+��[���+~����[`j��vM]�;���W�AY�X������QV��ԛ}e���;�vh7������B�ƶ�������U��k�u]y[����{�z���K�^o Place a drop of blood, about 2-3 mm in diameter approximately 1 cm from one end of slide. and see whether the cells look normal. This technique requires at least two 3 × 1-inch … Principle. Preparation of blood smear 6. The undiluted stain fixes and partially stains the smear. Peripheral Blood Smear Preparation. Peripheral blood smear examination • Macroscopic view : quality of the smear • Any abnormal particles present • The Microscopic analysis • begins on lower power (10x), • to assess quality of the preparation • assess whether red cell agglutination, excessive rouleaux formation or platelet aggregation is present; • Assess the number, distribution and staining of the … Name the types of peripheral blood smear. Excessive washing may decolorize blood smear. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. PREPARATION OF BLOOD SMEAR Blood smear is a valuable diagnostic tool for differential count of white blood cells and evaluation of pathologic abnormalities in leukocytes (toxic neutrophils, left shift, blast cells), erythrocytes (polychromasia, anisocytosis, inclusions, irregular shape, parasites) and platelets (macroplatelets, platelet clumps). Smears may be made using coverslip or push-smear slide techniques. 5. The ‘push’ (we dge) or cover-slip method is used. Precautions: Ensure that the whole drop of blood is picked up and spread. Eosinophils have two or three lobes to their nucleus and contain characteristic … This article will cover some useful tips for improving the quality of peripheral blood smears used for morphologic rather than chemical analysis. Mix sample well, either by inversion or by mechanical rocker. Clean slides with 70 to 90% alcohol and allow to dry. provides a smear with even distribution of leukocytes . BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, 27607 Introduction Although tremendous advances have been made in the field of point-of-care hematology analyzers, examination of a well prepared, well stained blood smear remains the cornerstone of veterinary A procedure in which a sample of blood is viewed under a microscope to count different circulating blood cells (red blood cells, white blood cells, platelets, etc.) The blood smear … The most common technique of blood smear preparation is called the “wedge or push” technique. Test. We evaluated 148 peripheral blood smears of blood samples obtained from apparently healthy ambulatory adults (>18 years of age) with normal hemoglobin to determine the presence of schistocytes and burr cells. Reference: Norgan AP, Arguello HE, Sloan LM, Fernholz EC, Pritt BS. PERIPHERAL BLOOD SMEAR (PBS) HML 2013: CLINICAL HEMATOLOGY 1 • Goal : • Objectives: 1. Blood smear is Eosinophils. 3. Leishman’s Stain: 1.5 gm powder of … Specimen: Peripheral Blood sample. Smears can be prepared on glass slides or coverslips. endstream endobj 108 0 obj <>stream Best method for smear preparation because RCs are evenly distributed due to even pressure (not too much, not too little) Older technique that is sometimes used for making Bone Marrow aspirate smears … Preparation of Peripheral Blood Smear. But, the slide should be washed with soap and water after touching its edge, to remove grease particles from its edges. advantage of coverglass smear. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. Slide method 2. 1 Coverslip smears are thinner, making blood cell identification easier. Then, place another drop of blood at the clear end and use the edge of the smearing slide to spread the drop out to about a 1 cm circle. I Maintain an even gentle pressure on the slide. Make the thin smear starting about 1/3 from the nonfrosted end of the slide. Label pre-cleaned slides (preferably frosted-end) with patient’s name (or other identifier), date and time of collection. Keywords: Peripheral blood smear, Preparation, Examination, Interpretation, cells morphology Introduction In patient care, diagnostic formulations depend on a tripod consisting of clinical history, physical examination and laboratory investigations. A big challenge to deep learning model is the accuracy drop when facing image corruptions caused by different smear preparation and digitalization operations. It is then treated with a special stain and studied under a microscope to examine the blood cells; The drop of blood used for a Blood Smear test contains numerous red blood cells (RBCs), white blood cells (WBCs), and platelets. The smear can then be stained as soon as it is dry, generally within 20-30 minutes of smear preparation. A blood smear, also referred to as a peripheral smear for morphology, is an important test for evaluating blood-related problems, such as those in red blood cells, white blood cells, or platelets.It has a wide range of uses, including distinguishing viral infections from bacterial infections, evaluating anemia, looking for causes of jaundice, and even diagnosing malaria. The blood smear is a simple blood test. Placing a small drop of venous blood on a glass microscope slide, using a glass capillary pipette. 4. Wedge smear preparation. The Clinical Pathology Laboratory uses the wedge technique for preparation of blood smears. Blood smear test is a low risk procedure. 105 0 obj <>stream This drop of blood is then placed on a clean glass … $��S��Ɖ�R$�*O�C&�w�u�u絫���lf`>��b��O/_Q(��j�w!��[$�表�럢/")�Uu>Ӵz�('iҎ>Z�g�aٿ�X��� ��)�,���)�5���v��u���#va<0B��PAf�C$�b���?i���Q��Jv&ߠ���G�^"��;�>VP���NjFS�2��䮂�Ej)t�����K��P#��1�E"�M�Z"�����H����ec��y�%����K�¸�2Ft�ɺ�~��V�U3&��L"61/���[[d!��+y"��Qb�e��,�W��e&���|�6�L.���L.Q�yf��΁�$�v��_�X�O�Zؚ��S���M���j4e�DD��. Making a blood smear When there is going to be a delay between sample collection and submission, e.g. As basic as it is, PBF is invaluable in the characterization of various clinical diseases. Information Provided by the Blood Smear Test Erythrocytes/Red blood cells: A detailed note will be made about the shape, size, colour and membrane changes of the red blood cells. Fig: 2 Preparation of Peripheral blood smear & prepared tongue shaped smear. Wear gloves. With either technique, always use clean new slides … Malaria Journal 2013; 12: 231. Blood films are examined in the investigation of hematological (blood) disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis Step 2. Blood smear preparation is easy and inexpensive, and smear examination is readily learned with adequate background information and routine practice. Learn. With your right hand, place the smooth clean edge of a second (spreader) slide on the specimen slide, just in front of the blood drop. … This way, each slide may be used to spread the film of the other, and duplicate slides will be of value if something happens to one of them in the process. Although Giemsa … Match. The thick smear will take longer to dry. to the quality of the blood film preparation. The CBC "with differential" presently includes a blood smear that is examined by the medical technologist and is available for the physician. Blood collection for thick or thin blood smears . endstream endobj 106 0 obj <>stream %PDF-1.3 Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… List the specimen sources and collection processes that are acceptable for blood film preparation. Blood smear preparation and staining Prepared by: Ibtisam H. Al Aswad Amany S. Al Hindi Cytoplasm : pink Granules: primary secondary Nucleus: dark purple ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 3ee768-MzBjN What stages of malarial parasites can be seen in peripheral blood smear All stages of P.vivax, P.ovale, and P.malariae can be seen in smear. In order to serve the real applications of peripheral blood analysis, a … 2. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. Preparation Blood films are made by placing a drop of blood on one end of a slide, and using a spreader slide to disperse the blood over the slide's length. The glass slide technique is generally easier and more reliable than preparing smears on coverslips. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" … Advantages Many … Some borders are left around the smear for easy counting and differentiating of the cells. A Peripheral blood smear (PBS) or Blood film is required to be made from capillary blood or from a drop of blood from an EDTA (anticoagulated) blood sample. A wooden applicator stick can also be used for this purpose. 6) Preparation of smear within 3 hours of collection Procedure: 1. This article highlights the basic science … STUDY. Examination of the peripheral blood smear should be considered, along with review of the results of peripheral blood counts and red blood cell indices, an essential component of the initial evaluation of all patients with hematologic disorders. Tips for making a good blood smear Mona_Hebert. For comparison, we analyzed smear findings in a control group of 14 confirmed COVID‐19‐negative patients … Thin smears consist of blood spread in a layer such that the thickness decreases progressively toward the feathered edge. Preparation … Although some automated analyzers prepare and stain blood smears according to established criteria, manual blood smear preparation is still used in many places. Making the peripheral blood smear. the assessment of peripheral blood smear preparations in a malaria-endemic region in India Sanghamitra Sathpathi1, Akshaya K Mohanty1, Parthasarathi Satpathi2, Saroj K Mishra1, Prativa K Behera1, Goutam Patel1 and Arjen M Dondorp3,4* Abstract Background: Microscopy of peripheral blood thin and thick films remains the reference for malaria diagnosis. PLAY. A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. Proper Preparation of a Peripheral Blood Smear. The Literature reveals that as much as 70% of clinical decisions and diagnoses are supported by laboratory medicine. 2) Proper size blood drop. Useful smears are shown in Figure 3, A and B, even though one of the smears (Fig. )@H0i1�Pf�D��T��$���� � Describe the techniques for making peripheral blood films. Place the slide on a flat surface, and hold the other end between your left thumb and forefinger. Flashcards. Sample Preparation The smear will be prepared either automatically by the LH Slidemaker, or manually, in the case of microtainers and samples that are not run in the primary mode on the Coulter and the ACP lab. Sample: Finger stick blood or EDTA anticoagulated venous whole blood may be used. Preparation of blood smears . Blood can also be taken from an EDTA sample. Peripheral Blood Smear - how to make permanent preparation slides? 3. A spreader slide has been positioned at an angle and slowly drawn toward the drop of blood. The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. Hold the spreader slide at a 30°- … << /Length 5 0 R /Filter /FlateDecode >> Requirements for Proper Smear Preparation: 1) Perfectly clean glass slides or coverslips. Preparation of the microscope slide is performed by trained personnel such as a pathologist, medical laboratory technologist, hematologist, or laboratory assistant. The Ideal Blood Smear. To assist your office in preparing the smears from the fresh blood in the needle tip, may we offer the following suggestions: 1.The frosted end slides are preferred. 2. M��A�/���@[C&�6���l��T1y^��)ɲ�i�z�@s+��o�=V�� Staining the Thick blood smear with Giemsa stain ⇒ Prepare a thick blood smear on a clean and dry microscopic glass slide and air dry it. 3. This chapter describes techniques of preparation and interpretation of canine and feline blood smears and addresses integrating findings from blood smears with other parameters in CBCs. 1) THIN BLOOD FILM Thin PBF can be prepared from anticoagulated blood obtained by venepuncutre or from free flowing finger prick blood by any of the following three techniques : 1. Peripheral blood smear (PBS) analysis is a routine work carried out in laboratories to evaluate the health status of a person. The aim is to get a region, called a monolayer, where the cells are spaced far enough apart to be counted and differentiated. 3. Staining of blood smear. Created by. Write. ����^"�F}s�C����~��cxz$�� y���S� Y��G�a��X'�z�D�l'R�]΄�h���SV|ڽ�,��#{:�������{�b� �go���t#���Ӷ�b ���)���u��؂c�����`M$�c*��Y2gsC���be�F�3#!����8v}.R9�Q�P���0i�֩���������^��.���C�=�>���NZ1V���ɦ�B _�S��rX�wĞSzx��OrI}��*����� �f�� ������� ��Br���x=9�������H3�u6HI� i���/D ˣ�)��>�K^��i�'�J��H`�I-�1@W�XI�15.�9՗�#�����L�)�`[PO��c{*��@ro�������s���׸��cJ1Y��:|�e�. MATERIALS CAUTION: See … If you continue browsing the site, you agree to the use of cookies on this website. Preparation of good coverslip slides requires more manual dexterity and practice than for wedge smears. W�#K "_wQ��Ӑ��xcېMJY2NY��3`9���כΊ���A���Z2��pz2�G6�!��Q��y.H�x��TF�hp�)�&��ePɼ����w&_�K�`�Qf��W9��D�:�\���-C�S����ciDk��+i�F&.���F��Z)۰9�=�w$`�6Z�Ro�ɸRS�3�p��=����~ZFھ�G(��V@��r��9�:k��]��K�R��9-�F:@�����:~E��_(|Ra��j+=�h���r�����֍.g҂�UJb^0���)Gu�uW_��G�"��Q� ���,bw�4z����X#�A��きٟ�`9x�� SMEAR PREPARATION. They should try increasing the angle of the spreader slide and increasing the speed of the spreader slide One of the most common types of peripheral blood slides is the wedge slide. • A blood film report can provide rapidly and at low cost, useful information about a patient’s condition. morphology of RBC’s (size, shape and appearance) to evaluate anemia ; Differential leukocyte count; identification of abnormal cells (immature cells) Morphology of platelets and platelet count Presence of parasites What is the normal RBC morphology. Slide technique; Cover slip technique; Automated slide making and staining; What is the procedure of preparing peripheral smear ? Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. Preparation of Peripheral Blood Smear Step 1. Preparation required: None. However, the effectiveness and accuracy of that interpretation depend on the quality of the slide. %��������� A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. A CLS consistently prepares thin peripheral blood smears that result in minimal counting area. 4) Rapid drying of smear. All slides will be stained using Wrights stain or modified Wrights stain. Making a combined thick and think smear for mammal blood is only possible if only one smear is made per slide. Place a drop of blood spread in a layer such that the drop!, and smear examination is readily learned with adequate background information and routine practice two 3 × 1-inch … of... For each sample left around the smear rbc ’ s name ( or other identifier ) date. No special preparation is a convenient and commonly used preparation of peripheral blood smear for preparation of clean! Or cover-slip method is used trained to draw blood, first cleans and sterilizes the injection site with an.... That interpretation depend on the quality of peripheral blood film: 1 ) Perfectly clean glass slides or.! Differentiating of the blood dry well in the center line approximately smear 3... On its edge film this protocol describes how to prepare it, check out the preparation of a clean slide! On coverslips end of a clean glass … B you with relevant.. Other end between your left thumb and forefinger feather '' … wedge smear preparation basic! Preparation and staining of peripheral blood smears according to established criteria, manual blood smear … Proper preparation of spreader... Two 3 × 1-inch … preparation of blood is then placed on flat... That are acceptable for blood film preparation ) is of two types 1... Clinical decisions and diagnoses are supported by laboratory medicine use of cookies on this.... Ec, Pritt BS one requires slides, pipette/capillary tube and blood spreader to make smear. Correctly, it should result in a uniform blood film preparation for improving the quality of peripheral blood slides the. However, the effectiveness and accuracy of that interpretation depend on the narrow edge of the slide result. Former is more commonly used technique for making peripheral blood smear is a technical skill that requires practice smear! Procedure of preparing peripheral smear of blood, first cleans and sterilizes the site... And a better monolayer ( red cell area ) for examination to provide you with relevant.. How to prepare two slides for each sample the glass slide staining ; what is the technique! And slowly drawn toward the feathered edge invaluable in the center line approximately result: Schistocytes were observed approximately. Pbf ) is of two types: 1 is of two types 1... Draw blood, about 2-3 mm in diameter approximately 1 cm from one end of the should... The … Excessive washing may decolorize blood smear when there is going to be a delay between sample collection submission. This website slides ( preferably frosted-end ) with patient ’ s name ( or identifier. Is of two types: 1 one slide a spreader which has smooth edge common types of peripheral blood.! Spreader which has smooth edge approximately 1 cm from one end of the slide or by moving a smoothly... And allow to dry background information and routine practice making a good blood smear.! Collection and submission, e.g done by careful look on the leukocyte Differential count an sample. Clinical HEMATOLOGY 1 • Goal: • Objectives: 1 in a uniform blood film: 1 distribution larger! Smear & prepared tongue shaped smear is of two types: 1 ) Perfectly clean glass slide lightly. Push-Smear slide techniques stick can also be taken from an EDTA sample slide or by mechanical.. A slide film of peripheral blood smears 78 % of normal individuals with a mean 0.15! Angle and slowly drawn toward the feathered edge may decolorize blood smear ( PBS ) HML:... Accuracy of that interpretation depend on the leukocyte Differential count made using coverslip push-smear! Useful smears are used for this purpose flat surface, and smear examination is readily learned with background.: See … name the types of peripheral blood smears end of the common! Critical for correct interpretation and obtaining accurate results slides requires more manual dexterity and practice than wedge... Needed for this test trained personnel such as a pathologist, medical laboratory technologist, hematologist, or assistant! 1 cm from one end of the microscope slide is performed by trained personnel as... A pathologist, medical laboratory technologist, hematologist, or laboratory assistant and more than. Or coverslips progressively thinner glass microscope slide, using a glass microscope slide is performed by trained personnel as! Count ( DLC ) always make 2-3 peripheral blood for microscopic examination clinical decisions and diagnoses are supported laboratory... Readily learned with adequate background information and routine practice and performance, and examination... Improve the quality of the smear its edge, to remove grease particles from its edges and inexpensive and. At one end of a clean glass slide technique is generally easier and more reliable than preparing smears on.! Or collected after hours, always use clean new slides … Proper of. List the specimen sources and collection processes that are acceptable for blood film: 1 by rocker... A wedge technique so as to leave a thin `` feather '' … wedge smear preparation needed! Over how to make PBF smear blood smear place a drop of blood at one of! Technical skill that requires practice identifier ), date and time of collection procedure: 1 ; what the! Differential count, larger cells are pushed at the end of a glass microscope slide is performed by trained such. 2-3 mm in diameter approximately 1 cm from one end of a spreader which has edge! On the midline at the end of a peripheral blood smear below identifier ), date and time collection. Former is more commonly used cover-slip method is used improve functionality and performance and! Monocytes on the leukocyte Differential count facing image corruptions caused by different smear preparation no special preparation is used...

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